DETERMINATION OF LIPASE ACTIVITY

Determination of lipase activity.

Heretofore most methods for the determination of lipase activity have employed emulsions of fatty acid esters in water (1). These emulsions have been stabilized by use of such agents as gum acacia. The disadvantages of such methods are that (1) the emulsions break up to a considerable and variable extent during the course of the incubation, and (2) the enzyme is presumed to be in the aqueous ph...

Turbidimetric procedure for determination of lipase activity.

A method is described for turbidimetric assay of Iipase activity. The substrate is a two-phase emulsion composed of a fairly homogenous dispersion of micelles of either triolein or olive oil. The de-emulsification of the substrate after lipase addition is a true measure of the enzymatic activity. Turbidity change is related to amount of the enzyme by use of a commercial standard, purified lipas...

Determination of Lipase Activity by Reginald M. Archibald

Heretofore most methods for the determination of lipase activity have employed emulsions of fatty acid esters in water (1). These emulsions have been stabilized by use of such agents as gum acacia. The disadvantages of such methods are that (1) the emulsions break up to a considerable and variable extent during the course of the incubation, and (2) the enzyme is presumed to be in the aqueous ph...

Lipase Activity of Mycoplasma.

Enzymatic activity of lipase

The knowledge of the digestive system of bullfrogs is an important step for the determination of their nutritional requirements throughout growth phases. With the objective of evaluating the enzymatic activity of lipase in the intestinal content of bullfrogs (Rana catesbeiana Shaw, 1802), 100 animals with median weight of 3.6 g were distributed in stalls under controlled temperature and photope...